關於 hairpin rna ，我們在網路上蒐集到這些相關的討論、資訊與評價

#11. 小髮夾RNA - A+醫學百科

小髮夾RNA（英語：short hairpin RNA，縮寫shRNA）是一種形成急轉彎（hairpin turn）結構的RNA序列，可以經由RNA干擾（RNAi）使基因表現沉默化。shRNA可利用載體導入 ...

#12. hairpin rna - 英中– Linguee词典

大量翻译例句关于"hairpin rna" – 英中词典以及8百万条中文译文例句搜索。

#13. hairpin loop (mRNA) | Learn Science at Scitable - Nature

A hairpin loop is an unpaired loop of messenger RNA (mRNA) that is created when an mRNA strand folds and forms base pairs with another section of the same ...

#14. Short hairpin RNA(shRNA) - 서린바이오사이언스

shRNAs are produced as single-strand molecules of 50–70 nucleotides in length, and form stem–loop structures consisting of a 19-29 base-pair region of double- ...

#15. A highly efficient short hairpin RNA potently down-regulates ...

Here we report stable knockdown of human CCR5 by a short hairpin RNA (shRNA) in a humanized bone marrow/liver/thymus (BLT) mouse model.

#16. Targeting L1 cell adhesion molecule using lentivirus-mediated ...

... molecule using lentivirus-mediated short hairpin RNA interference ... L1CAM using a lentivirus-mediated small hairpin RNA (shRNA) led to ...

#17. Rolling circle amplification-mediated hairpin RNA (RMHR ...

Abstract. Long hairpin RNA (lhRNA) construct-induced gene silencing facilitates the study of gene function in plants and animals, but constructing multiple ...

#18. 3p-hpRNA - InvivoGen

3p-hpRNA is a 5' triphosphate hairpin RNA that was generated by in vitro transcription of a sequence from the influenza A (H1N1) virus, a single‑stranded ...

#19. High-Throughput Construction of Intron-Containing ... - PLOS

With the wide use of double-stranded RNA interference (RNAi) for the analysis of gene function in plants, a high-throughput system for ...

#20. RNA interference by expressing short hairpin RNA in the ...

We carried out RNA interference by expressing short hairpin RNA (shRNA) in the Ciona intestinalis embryo. For this purpose, we identified a ...

#21. Short Hairpin RNA (shRNA) Interference: Therapeutic ...

Short hairpin RNA (shRNA) are produced in the nucleus of transduced or transfected cells which lead to the formation of hairpin structures.

#22. Short Hairpin RNA (shRNA): Design, Delivery, and ...

Shortly after the cellular mechanism of RNA interference (RNAi) was first described, scientists began using this powerful technique to study gene function.

#23. Large-Scale, High-Throughput Validation of ... - SLAS Discovery

Short Hairpin RNA Sequences for RNA Interference. LAURENCE H. LAMARCQ,1 BRADLEY J. SCHERER ... (siRNAs)2 or short hairpin RNAs (shRNAs), expressed stably in.

#24. Small hairpin RNA as a small molecule sensor - EMBO Press

The nucleotide sequence of the siRNA and its cousin, the small hairpin RNA (shRNA), depends solely on the mRNA they target. Because of the linear nature of ...

#25. 665. Advantages of “Bifunctional” Short Hairpin RNA for RNA ...

Two classes of RNAi molecules have been developed; the oligonucleotide based short interfering RNAs (siRNAs) and the vector based short hairpin RNAs ...

#26. Induction of Gene Silencing by Hairpin RNA Expression in ...

Surprisingly, expression of the RNA hairpin also causes targeted DNA deletion during conjugation, although at low efficiencies, which suggests a possible ...

#27. Short Hairpin RNA (shRNA): Design, Delivery ... - ResearchGate

Request PDF | Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of Gene Knockdown | Shortly after the cellular mechanism of RNA ...

#28. Selective gene silencing by viral delivery of short hairpin RNA

RNAi in cells can be induced from intracellularly expressed short hairpin RNAs either by shRNAs or synthetic miRNAs [2, 42, 43]. The basic ...

#29. Short hairpin rna (shrna734) and use of same to positively ...

A potent short hairpin RNA (shRNA734) directed to human Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) improves the rate of gene-modified stem cell ...

#30. short hairpin RNA | McManus Lab

shRNA is a ribonucleic acid polymer that is designed based on the concepts garnered from the study of naturally-occurring hairpin RNAs ...

#31. Short hairpin RNA (shRNA) 2 | Editable Science Icons from ...

Free vector icon Short hairpin RNA (shRNA) 2 by BioRender. Get this free icon and browse thousands of scientific icons. Make scientific figures in minutes.

#32. Short hairpin RNA-mediated inhibition of measles virus ...

The expression of Rab9 GTPase mRNA and protein were assayed by RT-PCR and ... The siRNA can be delivered as synthetic siRNA or short hairpin RNA (shRNA).

#33. Short Hairpin RNA

An artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi). [ NCI ]. Synonyms:.

#34. Downregulation of human epidermal growth factor receptor 2 ...

Subsequently, three different short hairpin RNAs (shRNAs) targeting the human HER2 gene were designed, constructed and transfected into human ...

#35. Study of Dendritic Cell Development by Short Hairpin RNA ...

Hairpin RNA -Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor. Cell Line In vitro. J. Vis. Exp. (181),.

#36. Short hairpin RNA loop design for the facilitation of sequence ...

Following transcription, the loop segment enables the formation of a short hairpin RNA (shRNA) structure that can be recognized and ...

#37. Companies Short Hairpin RNA - bionity.com

Everything relating to Short Hairpin RNA: compilations of facts, news, background knowledge, product information and market trends can be found here in the ...

#38. Low-toxicity chitosan gold nanoparticles for small hairpin RNA ...

The research for the present study focused on the preparation of low-toxicity gold nanoparticles (AuNPs) attached with small hairpin RNA (shRNA) and on ...

#39. Lentiviral Short Hairpin RNA Silencing of Histadine ...

Lentiviral Short Hairpin RNA Silencing of Histadine Decarboxylase in Human Mast Cells · Rationale. Histidine decarboxylase (HDC) is a unique intracellular enzyme ...

#40. Robust, Reversible Gene Knockdown Using a Single ...

Drug-inducible activation of short hairpin RNAs provides a more finely ... Short hairpin RNA expression cassettes generated by PCR were ...

#41. Hairpin RNA: a secondary structure of primary importance

In this review, we have focused on cis-acting RNA hairpins in metazoa, which regulate histone gene expression, mRNA localization and translation. We also review ...

#42. Principles of RNAi and shRNA Design - Cellecta

Small hairpin RNAs (shRNA) are sequences of RNA, typically about 80 base pairs in length, that include a region of internal hybridization that creates a ...

#43. Transient Expression of Homologous Hairpin RNA Causes ...

Transient Expression of Homologous Hairpin RNA Causes Interference with Plant Virus Infection and Is Overcome by a Virus Encoded Suppressor of Gene Silencing.

#44. One-step cloning of intron-containing hairpin RNA constructs ...

construction methods. Keywords Hairpin RNA • Intron • Isothermal in vitro recombination • Phytoene desaturase 3 (PDS3) • RNA.

#45. RNAi Induced Gene Silencing Journey from Simple dsRNA to ...

Hairpin RNA construct. Further the construct was improved with a transgene expressing RNA that folds back and hybridizes with itself to form a structure like a ...

#46. Expression Analysis of Hairpin RNA Carrying Sugarcane ...

Expression Analysis of Hairpin RNA Carrying Sugarcane mosaic virus (SCMV) Derived Sequences and Transgenic Resistance Development in a Model Rice Plant.

#47. Size-Controllable Enzymatic Synthesis of Short Hairpin RNA ...

DNA sequences for synthesizing anti-GFP, and negative control short hairpin RNA nanoparticles (shRNA-NPs). The complementary DNA sequence for the promoter ...

#48. Intron-Containing Hairpin RNA Interference Vector for OBP8 ...

Intron-containing hairpin RNA (ihpRNA) gene silencing with multiple restriction ligation steps was initial followed by gateway cloning with ...

#49. Synthetic RNA Recognition Motifs That Selectively Recognize ...

The U1hpII RNA hairpin contains a relatively large 10-nucleotide loop ... which new designer hairpin RNA-binding proteins can be generated, ...

#50. Medical Definition of Short hairpin RNA - RxList

Definition of Short hairpin RNA. Medical Editor: Melissa Conrad Stöppler, MD. Reviewed on 3/29/2021. Short hairpin RNA: See: shRNA.

#51. Rational Design of Hairpin RNA Excited States Reveals Multi ...

Rational Design of Hairpin RNA Excited States Reveals Multi-step Transitions. 2022/03/21. Read the original · 上一篇：用力打开PIEZO: 肖百龙/李雪明团队 ...

#52. Mammalian shRNA Tools for RNAi - Addgene

Short hairpin RNA (shRNA): design, delivery, and assessment of gene knockdown. · A lentiviral RNAi library for human and mouse genes applied to an arrayed viral ...

#53. Hairpin RNA-induced conformational change of a eukaryotic ...

NMR and fluorescence ani- sotropy revealed that Nterm binding to hairpin RNAs is weak but that the binding affinity increases significantly upon ...

#54. A functional screen for optimization of short hairpin RNA ...

shRNAs are stem-loop RNAs that require cellular biogenesis pathways prior to RISC loading. shRNA expression systems based on retroviral vectors ...

#55. Lentiviral Delivery of Small Hairpin RNA Targeting Connective ...

Knockdown of CTGC was achieved by lentivirus delivery of small-hairpin RNA. Cell proliferation was measured by CCK8, cell cycle progression, and apoptosis by ...

#56. Short hairpin rna - SlideShare

Sh RNA • Small hairpin RNA or short hairpin RNA (shRNA) is an artificial RNA molecule with a tight hairpin turn that can be used to silence ...

#57. Large-Scale, High-Throughput Validation of ... - SAGE Journals

Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T: Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature ...

#58. Short Hairpin RNA Interference Therapy for Ischemic Heart ...

Here we hypothesize that short hairpin RNA (shRNA) interference therapy targeting PHD2 can be used for treatment of myocardial ischemia and ...

#59. <FONT face=Verdana>Construction of short hairpin RNA ...

Abstract:Objective：To study the inhibit effect of survivin short hairpin RNA on the survivin mRNA and protein expression of gallbladder ...

#60. Short Hairpin RNA Knockdown of the Androgen Receptor ...

In this study, we have designed AR-targeted short hairpin RNAs (shRNA) and examined their ability to inhibit ligand-independent activation of a ...

#61. Plum Pox Virus Coat Protein Gene Intron-hairpin-RNA ...

Constructs with self-complementary sequences separated by an intron produce “hairpin” RNA [intron-hairpin-RNA (ihpRNA)] structures that efficiently elicit ...

#62. Sheep 7SK promoter for short hairpin RNA expression

Keywords: 7SK promoter, RNAi, sheep, shRNA. Abstract. Background: Gene silencing mediated by small interfering RNA (siRNA) has become a powerful ...

#63. single-step mixing cloning method for assembly of lentiviral ...

Lentiviral expression vectors encoding short hairpin RNA (shRNA) are widely used for RNAi-based gene silencing in mammalian cells.

#64. shRNA Process and Diagram - Sigma-Aldrich

What is shRNA? Vector-based short hairpin RNA (shRNA) is a type of RNA interference (RNAi) technology leveraged to study the function of unknown genes.

#65. A Novel Approach Obtaining Intron-Containing Hairpin RNA ...

Recently, much research on constitutive expression of an intron-containing self-complementary hair-pin RNA (ihpRNA) have been reported to silence target ...

#66. Bifunctional short hairpin RNA (bi-shRNA) - Semantic Scholar

The discovery of RNA interference (RNAi) engendered great excitement and raised ... We have developed a novel vector-driven bifunctional short hairpin RNA ...

#67. Short hairpin RNA expression for enhancing the resistance of ...

Keywords: RNA interference (RNAi), Silkworm Bombyx mori (Bm) cell line, short hairpin RNA (shRNA), Bm nucleopolyhedrovirus (BmNPV), ...

#68. 就立即加入 Facebook 吧。

A small hairpin RNA or short hairpin RNA (#shRNA) is a sequence of RNA that makes a tight hairpin turn that can be used to silence target gene expression...

#69. Short-hairpin (shRNA) Silencing AAV and Adenovirus

shRNA is an artificial RNA molecule that is used for gene silencing or RNA interference. Low degradation rate and turnover makes it ideal for gene therapy.

#70. Cold Spring Harbor Laboratory engages Hairpin ...

Cold Spring Harbor Laboratory engages Hairpin Technologies Inc. to license its short hairpin RNA (shRNA) technology. Tuesday, 31 March 2015.

#71. short hairpin RNA (shRNA) based allogeneic CAR T - Orphanet

The portal for rare diseases and orphan drugs · Search for an orphan drug · short hairpin RNA (shRNA)-based allogeneic CAR T.

#72. Short Hairpin RNA Construction - Bio-protocol

Got it! Short hairpin RNA (ShRNAs) targeting four distinct regions within the coactosin coding sequence were tested ...

#73. Review Hairpin RNA: a secondary structure of primary ...

Hairpin structures differ in size and localization within a messenger RNA (mRNA) sequence. Several examples of well-studied stem-loop structures and their ...

#74. Short Hairpin RNA–mediated Selective Knockdown of NaV1.8 ...

Short Hairpin RNA–mediated Selective Knockdown of NaV1.8 Tetrodotoxin-resistant Voltage-gated Sodium Channel in Dorsal Root Ganglion Neurons. Maya Mikami, M.D.;.

#75. Short-Hairpin RNA-Mediated Gene Expression Interference in ...

RNA interference (RNAi) is rapidly becoming a valuable tool in biological studies, ... By contrast, the expressed short-hairpin RNAs induce long-term, ...

#76. 小髮夾RNA

小髮夾RNA（英語：short hairpin RNA，縮寫shRNA）是一種形成急轉彎（hairpin turn）結構的RNA序列，可以經由RNA干擾（RNAi）使基因表現沉默化。shRNA可利用載體導入 ...

#77. RNA Interference from Biology to Therapeutics

Retrovirology 7:52 Schopman NC, Braun A, Berkhout B (2012) Directed HIV-1 evolution of protease inhibitor resistance by second-generation short hairpin RNAs ...

#78. What Is shRNA (Short-hairpin RNA)? - Genetic Education

"The shRNA is an artificial dsRNA loop or hairpin applicable in the gene silencing studies which rely on the mechanism of RNA interference."

#79. 小髮夾RNA - NiNa.Az

小髮夾RNA 英語short hairpin RNA 缩写shRNA 是一種形成急轉彎hairpin turn 結構的RNA序列可以經由RNA干擾RNAi 使基因表現沉默化shRNA可利用載體導入 ...

#80. OligoAnalyzer Tool - Primer analysis and Tm Calculator | IDT

Design and analyze DNA and RNA oligos for insight into behavior and properties. ... Predict oligo secondary structure or possible duplexes with HAIRPIN, ...

#81. 小发夹RNA介导的基因沉默及其应用 - 肿瘤防治研究

摘要 引言发夹结构的RNA(hairpin RNA)是RNA二级结构中最简单的。它是由一条RNA单链自身折叠,形成双链的茎,再加上一条单链的环构成,因此,发夹结构RNA又称为茎-环结构RNA ...

#82. RNA Towards Medicine - 第 156 頁 - Google 圖書結果

H1 RNA–pol III-based shRNA expression vector has been used to produce hairpin RNA with a 19-nt stem and a short loop (Brummelkamp et al. 2002a; Fig. 3).

#83. shRNA - - Celyad Oncology

Short hairpin RNA (shRNA) technology offers a differentiated strategy to future development of CAR T-cell therapies. Manipulating protein expression to ...

#84. RNA Interference in Practice: Principles, Basics, and ...

Add 15 worms to the RNA-liposome mixture; this results in a total volume of 15 μl and a final ... 2.14 Scheme ofthe hairpin RNA expression from an inverted.

#85. 小髮夾RNA - 中文百科全書

shRNA是short hairpin RNA 的縮寫。翻譯為“短髮夾RNA”。shRNA包括兩個短反向重複序列，中間由一莖環（loop）序列分隔的，組成髮夾結構，由pol Ⅲ啟動子控制。

#86. Retraction | Science

Hairpin RNAs and Retrotransposon LTRs Effect RNAi and Chromatin-Based Gene Silencing · RNAi Extends Its Reach · RNA Polymerase II Is Required for ...

#87. Structure, Functions and Types of RNA - Byju's

RNA resembles a hairpin structure and like the nucleotides in DNA, nucleotides are formed in this ribonucleic material(RNA). Nucleosides are nothing but the ...

#88. Nanoparticles-mediated circROBO1 silencing to inhibit HCC

Mechanistically, circROBO1 acted as a competing endogenous RNA to ... down circROBO1 HCCLM9 cells were constructed using short hairpin RNAs, ...

#89. Hairpin model of t-RNA - Doubtnut

Step by Step answer for “ Hairpin model of t- RNA ” of Biology Class 12th. Get FREE solutions to all questions from chapter .

#90. Editing the cell's transcripts | Drug Discovery News

The protein RNA polymerase binds to DNA and creates an RNA transcript from it. ... Single position substitution of hairpin pyrrole-imidazole ...

#91. Gene Knockdown Market Sales to Top USD ... - EIN News

This technique involves the use of small interfering RNA (siRNA) or short hairpin RNA (shRNA) molecules that bind to specific messenger RNA ...

#92. Recent advances in the study of sepsis-induced depression

... virus expressing short hairpin RNA targeting Cldn5, the peripheral ... a stress-induced decline in cFos mRNA and protein levels in the ...

#93. Primer3 Input

TH: Max Self Complementarity · TH: Max 3' Self Complementarity · TH: Max Pair Complementarity · TH: Max 3' Pair Complementarity · TH: Max Primer Hairpin ...

#94. JASPAR - A database of transcription factor binding profiles

... T-Box factors, TATA-binding proteins, TEA domain factors, Tryptophan cluster factors, Zipper-type, beta-Hairpin exposed by an alpha/beta-scaffold, mTERF ...

#95. Ovalbumin - p01012 · oval_chick - UniProt

The hydrophobic sequence, which lies between residues 27 and 43, folds back on the preceding residues to form an amphipathic hairpin structure which is the ...

#96. Linewidth in matlab - City bei Engin in Uhldingen

The linewidths of the H8 resonances of the deuterated RNA hairpin are 20−25% narrower ... MATLAB Functions, Commands, and Operators Covered in This Chapter ...

#97. Ryuya Fukunaga Ph D Associate Professor of Biological ...

He completed postdoctoral training in RNA silencing mechanism at the University of ... derived from genome encoded long hairpin RNA or overlapping mRNAs.

#98. DOI: 10.1126/science.1089910 , 1023 (2004); 303 Science et ...

Cdh1 RNA interference in neurons was also found to override the inhibitory ... of cdh1 small hairpin RNAs (shcdh1) reduced. Cdh1 expression in COS cells and ...

#99. Oral Cancer: New Insights for the Healthcare Professional: ...

Graduate Institute of Life Sciences, Taipei: Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral ...